AB0064 DISCOVERY OF NOVEL LONG-NONCODING RNAS IMPLICATED IN RHEUMATOID ARTHRITIS

Background Rheumatoid arthritis (RA) is a representative systemic autoimmune disease characterized by synovial inflammation and proliferation accompanied joint destruction. Fibroblast-like synoviocytes (FLS) are key constituent of the synovium paly an important role in pathogenesis RA. Although accumulating evidence has implicated long non-coding RNAs (lncRNAs) diseases, lncRNAs RA remains unknown. Objectives In this study, we aim to investigate expression profile FLS from patients with (RA FLS) discover promising candidates involved Methods The next-generation sequencing (NGS) data were analyzed identify differential expressed compared osteoarthritis (OA FLS). NGS also reveal differentially between unstimulated IL-1-stimulated FLS. And then, intersection two analysis results was obtained. Data obtained validated RT-qPCR using OA Changes lncRNA induced pro-inflammatory cytokines investigated RT-qPCR. Results According analysis, among whose increased FLS, there 4 IL-1 stimulation On other hand, decreased 6 stimulation. Of upregulated downregulated lncRNAs, top 3 each when listed order fold change. As result, it confirmed that only one (NR_030732) level NR_030732 more enhanced after treating IL-1, IL-6, or TNF-α not validation, (NR_034107, NR_046240, NR_121608) derived treatment Conclusion Our study found prominently cytokines. Thus, our suggest might be candidate Reference [1]Hur K, Kim SH, JM. Potential Implications Long Noncoding Autoimmune Diseases. Immune Netw. 2019 Feb 25;19(1):e4. Acknowledgements: NIL. Disclosure Interests None Declared.